Journal: iScience

Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease

doi: 10.1016/j.isci.2026.117636

Figure Lengend Snippet:

Article Snippet: Mouse anti-Synaptophysin (SYN) , Santa Cruz Biotechnology , Cat# sc-365488.

Techniques: Model Organisms, Recombinant, CCK-8 Assay, Apoptosis Assay, ROS Assay, Calcium Assay, Enzyme-linked Immunosorbent Assay, Generated, Software, Genotyping

Journal: Life

Article Title: Ascorbic Acid Neuroprotection Against Hippocampal Injury and Gliosis Induced by E621 in Albino Rats Through Modulation of GFAP, Synaptophysin, and Caspase-3

doi: 10.3390/life16081234

Figure Lengend Snippet: Representative photomicrographs of synaptophysin-immunostained sections in the DG of all studied groups. Synaptophysin appears minimal in the GL (arrow) of G1 and G2; however, it is significantly elevated, with robust, prominent cytoplasmic expression (arrows) in the GL of G3. In contrast, synaptophysin is significantly reduced in the GL (arrows) of G4 compared with G3. (synaptophysin immunostaining 400×).

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP 1:100 (MS-280-B0; Lab Vision Corporation; Fremont, CA, USA), synaptophysin (A6344; Abclonal Technology; 1:100), and caspase-3 1:100 (PA1-29157; Thermo Fisher Scientific; Waltham, MA, USA).

Techniques: Expressing, Immunostaining

Journal: Life

Article Title: Ascorbic Acid Neuroprotection Against Hippocampal Injury and Gliosis Induced by E621 in Albino Rats Through Modulation of GFAP, Synaptophysin, and Caspase-3

doi: 10.3390/life16081234

Figure Lengend Snippet: M ± SEM of the percentage of degenerated neurons and the immunoreactivity of GFAP, synaptophysin, and activated caspase-3 in the DG region of the hippocampus across all groups.

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP 1:100 (MS-280-B0; Lab Vision Corporation; Fremont, CA, USA), synaptophysin (A6344; Abclonal Technology; 1:100), and caspase-3 1:100 (PA1-29157; Thermo Fisher Scientific; Waltham, MA, USA).

Techniques:

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effect of ozone exposure on tumor cells was studied by measuring ROS levels in B16F10 melanoma and LL2 lung carcinoma cells. (A) The ROS content was assessed using the ROS-ID Total ROS Detection Kit. Data from the 520 nm fluorescence indicates increased ROS levels. The increase in ROS in (B) B16F10 melanoma and (C) LL2 lung carcinoma cells was observed after three days of treatment with air or ozone, with or without arbutin (1.56 µM). These measurements were performed using the Chekine TM reactive oxygen species detection fluorometric assay. (D) Cell proliferation of B16F10 melanoma and LL2 lung carcinoma was evaluated following three-day ozone exposure, revealing enhanced proliferation in both cell lines. Results are shown as mean ± SD with a sample size of 6; statistical significance is indicated by *P < 0.05, **P < 0.01, and ***P < 0.001. Western blot images display the expression of PCNA and HIF-1α proteins in (E) B16F10 (F) LL2 cells exposed to ozone or air at various time points.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: Fluorescence, Western Blot, Expressing

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The effects of ozone on the HIF-1α/CXCR4 pathway and EMT markers in B16F10 and LL2 in vitro models. Western blot images showing levels of HIF-1α, CXCR4, E-cadherin, N-cadherin, MMP-2, and SNAIL proteins. Inset values represent protein expression levels normalized to β-Actin.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro, Western Blot, Expressing

Journal: American Journal of Cancer Research

Article Title: Reactive oxygen species-dependent regulation of hypoxia-inducible factor 1α/C-X-C motif chemokine receptor 4 signaling promotes ozone-induced cancer metastasis

doi: 10.62347/DKDS6537

Figure Lengend Snippet: The ROS-related effects of in vitro arbutin application on ozone-exposed EMT depolarization and HIF-1α/CXCR4 pathway downregulation in (A) B16F10 and (B) LL2 cells. Protein expressions of HIF-1α, CXCR4, E-cadherin (E-Cad), N-cadherin (N-Cad), MMP-2, and SNAIL are shown with arbutin-reversal treatment. Inset values indicate protein levels normalized to β-Actin. Each experiment was repeated three times with similar results.

Article Snippet: The HIF-1α inhibitor (SYP-5) was purchased from MedChemExpress (MedChemExpress LLC, Monmouth Junction, NJ, USA).

Techniques: In Vitro